Journal of Research in Biology
JRB
Biology
http://jresearchbiology.com/
0
2231–6280
2231- 6299
en
2017
09
27
7
6
online
1
fulltext
en
Comparative evaluation of hyaluronic acid production by Streptococcus thermophilus isolated from yoghurt
Biology
Original Research
Hyaluronic acid (HA) is also known by the name hyaluronan. The necessity for using this fabulous material lead to investigate non-pathogenic strains which produce this material. The most non-pathogenic strain is S. thermophilus. The lack of literature on microbial production of this substance by the strain prompted us to examine the microbial production of HA from it and also to examine optimization of culture conditions where HA is produced. The bacteria Streptococcus salivarius sub. thermophilus was obtained from the Bank of Scientific and Industrial Research of Iran (PTCC 1738). To separate S. thermophilus strains from yogurts, three types of yogurts were used. They were cultured by pour-plate and surface methods on STA medium. To identify the isolated strains, biochemical tests and Polymerase Chain Reaction (PCR) were used. Bacterial strains isolated from yoghurts were identified as S. thermophilus MN-BM-A02, S. thermophilus JIM8232 and S. thermophilus MN-ZLW-002. To separate the capsule strains, each strain was cultured on STB medium and then they were centrifuged. In order to purify the samples, ethanol and charcoal were used. To optimize production, variety of sources of carbon, nitrogen, temperature and pH were studied
Hyaluronic acid, Streptococcus thermophilus, FTIR
2342
2354
http://jresearchbiology.com/documents/RA0620.pdf
Diba
Tabatabaei
No
Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abbas
Akhavan
Sepahy
Yes
Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran