Journal of Research in Biology
JRB
Biology
http://biologyresearch.info/
0
2231-6280
2231-6299
en
2015
05
26
4
4
online
8
fulltext
en
In Vitro rapid multiplication of Pergularia daemia - A medicinal plant
Biology
Original Research
A protocol for in vitro multiple shoot development and plantlet formation using leaf explant was prescribed for Pergularia daemia (Forssk.) Chiov. medicinally important plant species. Young apical leaf explant was used for callus induction on MS medium containing TDZ and NAA at 1.5 and 0.2 mgl-1 respectively showed maximum callus induction (77%). The amount of callus responded for shoot formation (75%) was obtained in the MS medium containing IBA (1.5 mgl-1) and TDZ (0.3mgl-1). The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.3 mgl-1) for shoots rooted. Regenerated plantlet were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate
Pergularia daemia, MS medium, TDZ, Multiple shooting, Acclimatization
1732
1737
http://jresearchbiology.com/documents/RA0526.pdf
Femila
Jose
No
PG and Research Department of Botany, Scott Christian College, Nagercoil - 629 003
Asir
Selin
Kumar
Yes
University of Science and Technology of Masuku, National Higher Institute of Agronomy and Biotechnology (INSAB). Poto-Poto BP 99 Franceville, Gabon